Coding

Part:BBa_K2923009:Design

Designed by: Kateryna LEN   Group: iGEM19_Strasbourg   (2019-10-13)

LexA WT repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein specifically recognizes the LexA operator sequence, which contains a wild-type half-site (CTGT). LexA WT repressor did not contain stop-codon, which allows its fusion to analyze protein at Cter.


Bibliographic sources

Dimitrova, M., Younès-Cauet, G., Oertel-Buchheit, P., Porte, D., Schnarr, M., and Granger-Schnarr, M. (1998). A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli. Molecular and General Genetics MGG 257, 205–212.

Daines DA, Granger-Schnarr M, Dimitrova M, Silver RP. 2002. Use of LexA-based system to identify protein-protein interactions in vivo. Methods Enzymol 358:153–161.

Daines DA, Silver RP. 2000. Evidence for multimerization of Neu proteins involved in polysialic acid synthesis in Escherichia coli K1 using improved LexA-based vectors. J Bacteriol 182:5267–5270